Focus

rqmicro consists of a highly interdisciplinary team to develop and market innovative solutions. We focus on choosing and adapting suitable technologies to solve current problems with microbiological contamination in the water- and food industry. At the moment we focus on immunomagnetic separation in combination with microfluidic technology.


Biology

rqmicro’s scientific team studies bacteria that live in water and food and develops specific antibodies which are used for the detection. We develop our own antibodies to meet the high requirements of our rapid tests.


Immunomagnetic separation and Microfluidics

rqmicro uses the great potential of microfluidics to deliver a product, that allows target cells to be separated from a sample with high efficiency, using immunomagnetic separation (IMS). In contrast to conventional IMS methods, our approach separates target cells in a continuous process on a disposable cartridge, resulting in high recovery rates and easy handling.

Conventional IMS systems are labor intensive and lead to lower purity – especially in natural samples, where contaminants have a higher tendency to get stuck in the column. rqmicro’s microfluidic IMS process is contact-free and continuous, achieving highest purity rates on the market.

rqmicro’s synthesis of technologies for rapid on-chip sample separation

Immunomagnetic Separation rqmicro

 


Flow Cytometry

Flow cytometry is a laser-based electronic cell counting technology that allows multiparametric analysis of the properties of thousands of cells per second. The quantification of the cells in a given sample can be performed within minutes. Since it is a cultivation-independent method, all bacteria, also dormant or dead ones, are quantified. By using fluorescent viability dyes, we can discriminate viable from dead cells. This is especially useful when tracking efficacies of disinfection measures. These facts lead to superior data in almost real time.

two fluorescence detection cartoon-01

 


Publications

Keserue, H.-A., Bertsch D., Schaffhauser D. Detektion von Legionellen in nur einer Stunde. WWT3: 8-11 (2014).

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Keserue, H.-A., Bertsch D., Schaffhauser D. Schnelldetektion von Legionellen. Aqua & Gas 11: 36-39 (2013).

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Keserue, H.-A., Baumgartner, A., Felleisen, R., Egli, T. Rapid detection of total and viable Legionella pneumophila in tap water by immunomagnetic separation, double fluorescent staining and flow cytometry, Microb. Biotechnol. 5: 753-763 (2012).

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Keserue, H.-A., Füchslin, H. P., Wittwer, M., Nguyen-Viet, H., Nguyen, T.T., Surinkul, N., Koottatep, T., Schürch, N. and Egli, T. Comparison of rapid methods for detection of Giardiaspp. and Cryptosporidium spp. (oo)cysts using transportable instrumentation in a field deployment. Environ. Sci. Technol. 46: 8952–8959 (2012).

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Keserue, H.-A. & Egli, T. Durchflusszytometrie: Nachweis von Krankheitserregern. Aqua & Gas 5: 34-38 (2012).

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Keserue, H.-A., Füchslin, H. P. & Egli, T. Rapid detection and enumeration of Giardia lamblia cysts in water samples by immunomagnetic separation and flow cytometric analysis. Appl. Environ. Microbiol. 77: 5420-5427 (2011).

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Füchslin, H. P., Kötzsch, S., Keserue, H.-A. & Egli, T. Rapid and quantitative detection of Legionella pneumophila applying immunomagnetic separation and flow cytometry. Cytometry 77A: 264-274 (2010).

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